rockbio.io · Bioinformatics

Production bioinformatics server — Ubuntu 24.04 LTS

● All systems operational

Server

OS
Ubuntu 24.04 LTS
CPU cores
8
RAM
31Gi
Disk (total)
5.4T
Disk (free)
5.0T
Python
3.11.15
R
4.3.3
Last updated
2026-04-28 12:06 UTC

Alignment & Mapping

BWA
Burrows-Wheeler Aligner
BWA-MEM2
Faster BWA for modern CPUs
2.2.1
Bowtie2
Fast short-read aligner
2.5.5
HISAT2
Splice-aware RNA/DNA aligner
2.2.2
STAR
Spliced Transcripts Alignment
2.7.11
minimap2
Long-read & splice aligner
2.30
subread
Subread aligner
2.1.1 —

Quality Control & Trimming

FastQC
Per-base sequence quality
0.12.1
MultiQC
Aggregate QC reports
1.34
fastp
Ultra-fast FASTQ preprocessor
1.3.3
Trimmomatic
Flexible read trimmer
0.40
Trim Galore
Trim + FastQC wrapper
0.6.11
Falco
Fast FastQC alternative
1.3.0
samtools
SAM/BAM/CRAM processing
1.23.1
picard
Broad SAM/BAM toolkit
3.4.0 —
sambamba
Fast samtools alternative
1.0.1
mosdepth
Fast coverage depth
0.3.14

Variant Calling

GATK4
Genome Analysis Toolkit
4.6.2.0
bcftools
Variant calling & manipulation
1.23.1
FreeBayes
Bayesian variant caller
1.3.10

Structural Variants & Copy Number

Manta
SV caller (Illumina) — Docker
1.6.0
DELLY
SV discovery paired-end
1.7.3
CNVkit
Copy number variation toolkit
3.11 —
mosdepth
Depth of coverage
0.3.14

Annotation

SnpEff
Variant annotation & effect
5.2
vcfanno
Annotate VCF with any file
0.3.7 —

RNA-seq & Expression

Kallisto
Pseudoalignment quantification
0.52.0
Salmon
Transcript quantification
0.13.1
StringTie
Transcript assembly & quant
3.0.3
HISAT2
Splice-aware RNA aligner
2.2.2
STAR
RNA-seq aligner
2.7.11

Methylation

Bismark
Bisulfite mapping & extraction
0.25.1

Long Reads

minimap2
Long-read aligner
2.30
NanoStat
Nanopore read statistics
1.6.0
Chopper
Nanopore quality filtering
0.11.0
seqtk
Sequence toolkit

Assembly

SPAdes
De novo genome assembler
4.2.0
Flye
Long-read assembler
2.9.6

Metagenomics

Kraken2
Taxonomic classification
2.17.1
Bracken
Abundance re-estimation
3.0.1
Diamond
Fast protein alignment
2.1.24

Utilities

bedtools
Genome arithmetic
2.31.1
seqkit
FASTA/Q toolkit
002516.2 —
vcftools
VCF file processing
0.1.17
PLINK2
GWAS analysis toolkit
2.0.0
SRA-tools
NCBI SRA download tools
2.8.0
Entrez-Direct
NCBI Entrez CLI
24.0
pigz
Parallel gzip
2.8
GNU parallel
Shell job parallelisation

Workflow Managers

Nextflow
DSL2 pipeline framework
25.10.4
Snakemake
Python-based workflow manager
9.19.0

R & Bioconductor

DESeq2
Differential expression for count data
1.42.1
edgeR
Differential expression (NB model)
4.0.16
limma
Linear models for omics data
3.58.1
GenomicRanges
Genomic interval manipulation
1.54.1
Biostrings
Biological sequence manipulation
2.70.3
Rsamtools
BAM/SAM file interface
2.18.0
rtracklayer
Import/export genomic tracks
1.62.0
AnnotationHub
Bioc annotation resources
biomaRt
Ensembl BioMart interface
2.58.2
SingleCellExperiment
Single-cell infrastructure
1.24.0
scran
Single-cell normalisation
1.30.2
VariantAnnotation
VCF parsing & annotation
1.48.1
minfi
DNA methylation arrays
1.48.0
ChIPseeker
ChIP-seq peak annotation
DiffBind
Differential binding analysis
clusterProfiler
Gene ontology & enrichment
ComplexHeatmap
Complex heatmap visualisation
2.18.0
EnhancedVolcano
Publication volcano plots
1.20.0
Gviz
Genomic data visualisation

Services

RStudio Server
R IDE in the browser
Open →
JupyterHub
Multi-user Jupyter notebooks
Open →
AI Hub
Open WebUI, LibreChat, Flowise
Open →
Crypto Dashboard
Trading strategy bot
Open →